Services
Summary of our expertise and services
We are expert in the early stage of biodiscovery and are happy to share this expertise with you. Our services cover both analytical chemistry and screening of fractions / extracts / pure compounds for bioactivities. We can perform dereplication and analysis of active biomaterial using our brand new Xevo UHPLC-HR-MS2 as well as fractionate your sample and isolate pure compound from complex mixture.
We also offer a wide selection of activity assay ranging from anti-cancer, antibacterial to ADME characteristics for the more promising hits (see table below).
Assay categories available at Marbio:
|
Assay category |
Assay type |
Assay principle / name |
Details / cells lines |
| Anti-cancer | Cell viability | MTS, Cell titer Glo | Cell line available: MCF-7 (breast cancer); HT-29 (adenocarcinoma); MV4-11 (AML); A2058 (Melanoma); Ri-1 (B-cell lymphomas); MDA-MB-468 (TNBC); NALM6 (ALL); and other upon request |
| Antimicrobial | Growth inhibition | OD600 | Microbial strains available: E. Faecalis, E. Coli, P. Aeruginosa, S. Aureus, S Agalactiae. |
| Antimicrobial | Biofilm inhibition | Crystal violet staining | S. Epidermidis |
| Anti-Inflammatory | ELISA | Detection of secreted TNF-α | Using Thp-1 cells (monocytes) treated with LPS. Possibility to detect cytokine IL-1β too |
| Immunostimulatory | ELISA | Detection of secreted TNF-α | Using Thp-1 cells (monocytes) treated with LPS. Possiblity to detect cytokine IL-1β too |
| Antioxidant | Biochemical | ORAC | Measuring degradation of fluorescein by a radical forming molecule (AAPH) |
| Antioxidant | Chemical | FRAP | Reduction of FeIII complex to FeII leading to colorimetric / absorbance changes in solution |
| Toxicity | Cell viability | MTS | Using healthy lung fibroblast-like cells: MRC-5 and liver cancer cell lines HepG2 |
| ADME | Aqueous solubility | SOLRANK | Kinetic solubility of compound in aqueous buffer compared to acetonitril |
| ADME | Membrane permeability | PAMPA | Permeability of compound through artificial lipid membrane |
| ADME | Metabolic stability | Mouse liver microsome | Digestion of compound by mouse hepatic enzymes |
The assays above are performed routinely in our lab, however we are familiar with a broad range of other assays, techniques and additional cell lines and protocols. For more information contact us directly at jeanette.andersen@uit.no
